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KMID : 1094720120170020385
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 2 p.385 ~ p.392
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Enzymatic synthesis of fructooligosaccharides from date by-products using an immobilized crude enzyme preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033
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Smaali Issam
Jazzar Souhir
Soussi Asma
Marzouki M. Nejib
Muzard Murielle
Aubry Nathalie
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Abstract
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Aqueous extracts from date by-products of the sucrose-rich variety “Deglet Nour” were used as a starting substrate to achieve the enzymatic synthesis of fructooligosaccharides (FOS) commonly used as prebiotics. A crude β-fructofuranosidase (Ffase) preparation from Aspergillus awamori NBRC4033 was immobilized on chitosan by covalent binding through glutaraldehyde linkages (Yi = 88%, Ya = 54%), and used for this purpose. The effect of water-extraction volume on the FOS synthesis by transfructosylation was studied. It was found that 150 mL/100 g of date by-products gave the best FOS concentration and productivity (123 g/L and 18.5 g/h/100 g respectively), related to an optimal sucrose conversion of 53.26%. The main FOS product was purified via a biogel-P2 gel filtration column. Its structure was determined as 1-kestose: α-Dglucopyranosyl-( 1→2)-β-D-fructofuranosyl-(2→1)-β-Dfructofuranoside by combination of 1H, 13C and 2D-NMR techniques. Our results provide new insights into the enzymatic synthesis of FOS from an alternative source of sucrose, namely date by-products.
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KEYWORD
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Aspergillus awamori, date by-products, fructooligosaccharides, β-fructofuranosidase, immobilization
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